Description
Dual Wavelength UV Absorption Analyzer
The AF46 is a dual wavelength, high precision UV light absorption analyzer designed for inline operation to provide accurate concentration measurements with remarkable repeatability, linearity and resolution. Wavelength and optical path length can be individually configured to specific requirements for the highest accuracy.
Used For
- UV range:
- 0 – 0.05 to 2 CU (concentration units), dependent on filter/OPL used
- UV absorption control and UV Absorption control
- in Chromatography: Pre / Post column monitoring (OD) for optimized pooling
- of concentrations in permeate or retentate
- in TFF / Ultrafiltration
- of organic load in water / condensate
- of the concentration of aromatics (Caprolactam, Aniline, Phenol, etc.)
- as TOC, COD, BOD trend analysis
- Disinfection control treatment of water
Typical Applications
Chromatography
Aromatics Detection
Ultrafiltration
Single-Use Technology
Leak Detection / Condensate / Carryover Monitoring
The AF46 is a dual wavelength UV analyzer ideally suited to monitor protein concentrations (e.g., monoclonal antibodies) in downstream biotechnology applications such as chromatography or filtration. Using two different wavelengths the sensor is capable of detecting very low protein concentrations for accurate pooling and at the same time is able to monitor the whole elution peak with very high optical density (OD) using a second wavelength.
Besides protein measurements the sensor is often used to monitor nucleic acid contaminations of protein solutions. Due to the modularity different optical path lengths (OPL) and wavelengths can be combined to measure any product absorbing in the UV range.
| AF46 | ||
|---|---|---|
| Schematic | 
|
|
| Measurement | ||
| Measurement principle | 2- Channel Absorption of light | |
| Measurement wavelength(s) | 254-13 / 280-13, 254-13 / 313-13, 280-09 / 300-05, 280-09 / 300-13, 280-09 / 313-13, 280-13 / 300-13, 280-13 / 313-13, 290-13 / 313-13 nm, … others on request | |
| Detector(s) | 2 silicon photodiodes (hermetically sealed) | |
| Reference detector(s) | 2 silicon photodiodes (hermetically sealed) | |
| Measuring Range | any measuring range between
0 – 0.05 to 2 CU (dependent on filter used / OPL) (contact product specialist for application specific ranges) |
|
| Optical path length | 1 – 160 mm | |
| Calibration | CU (concentration units) application specific calibration | |
| Light source | low pressure mercury lamp
typical life span: 1 to 2 years (8,000 to 16,000 hours) |
|
| Resolution | < ± 0.05 % of respective measuring range | |
| Repeatability | < ± 0.5 % of respective measuring range | |
| Linearity | < ± 1 % of respective measuring range (specific to application) | |
| Protection | all optical parts have an IP rating of IP65 or higher | |
| Sensor Body | ||
| Material | Stainless steel 1.4435 (SS 316L), 1.4539, 1.4571 (SS 316Ti), 1.4462, Titanium 3.7035 (Grade 2), Hastelloy 2.4602 (C22), Plastic TFM4215, PVC, … others on request | |
| Line size | 1/8 in. to 6 in. (DN 6 to DN 150), … others on request | |
| Process connection | Flanges (ASME, DIN, JIS), Clamps (TC, ISO, DIN), Female Threads (NPT, DIN), Sanitary Threads (DIN 11851), Tube Ends (DIN, ISO, OD), Varivent, … others on request | |
| Process pressure | 10 mbar to 100 bar (0.15 psi to 1450 psi) – higher on request depending on process connection, materials and design | |
| Windows | 2-Sapphire, 3-Sapphire Biotech (do not use Pyrex®) | |
| Window gaskets | Viton® (FDA), EPDM (FDA / USP Class VI), Kalrez® 4079, … others on request (do not use Silicone) | |
| Temperature Ratings | ||
| Process temperature | permanent: 0 – 70 °C ( 32 – 158 °F)
peak 15 min/day: 0 – 135 °C ( 32 – 275 °F) peak 30 min/day: 0 – 120 °C ( 32 – 248 °F) |
|
| Process temperature OPTION HT |
permanent: -30 – 120 °C (-22 – 248 °F)
peak 15 min/day: -30 – 150 °C (-22 – 302 °F) peak 30 min/day: -30 – 140 °C (-22 – 284 °F) |
|
| Process temperature OPTION EX |
permanent: -30 – 70 °C (-22 – 158 °F)
peak 15 min/day: -30 – 135 °C (-22 – 275 °F) peak 30 min/day: -30 – 120 °C (-22 – 248 °F) |
|
| Process temperature OPTION EX-HT |
permanent: -30 – 120 °C (-22 – 248 °F)
peak 15 min/day: -30 – 150 °C (-22 – 302 °F) peak 30 min/day: -30 – 140 °C (-22 – 284 °F) |
|
| Ambient temperature | permanent: 0 – 40 °C ( 32 – 104 °F)
operation: -30 – 40 °C (-22 – 104 °F) with options HT / EX / EX-HT transport: -20 – 70 °C ( -4 – 158 °F) |
|
| Explosion Proof | ||
| Ex-proof | none | |
| Ex-proof OPTION EX (EN-D) |
Sensor assembly in ex-proof version accor. ATEX (EN-D) | |
| Ex-proof OPTION EX (FM-D) |
Sensor assembly in ex-proof version accor. FM (FM-D) | |
| Calibration | ||
| Calibration adapter VB | Filter adapter FH03 for calibration filter used for sensor verification | |
| Pressure and temperature ratings specified herein may be subject to limitations – see instruction manual. The appropriate choice of material for all wetted parts is the sole responsibility of the user. Data given are subject to changes without prior notice. | ||
Product Variants
AF46-HT-VB
AF46-EX-HT-VB
Process temperature OPTION HT
permanent: -30 – 120 °C (-22 – 248 °F)
peak 15 min/day: -30 – 150 °C (-22 – 302 °F)
peak 30 min/day: -30 – 140 °C (-22 – 284 °F)
Ex-proof OPTION EX (EN-D)
Sensor assembly in ex-proof version accor. ATEX (EN-D)
Ex-proof OPTION EX (FM-D)
Sensor assembly in ex-proof version accor. FM (FM-D)
