Description
Single Channel Absorption Probe
The ASD12-N absorption probe has a durable LED light source for direct installation into laboratory bioreactors and disposable bioreactors for measuring cell growth and optical density. The probe is available in three different lengths to fit any vessel and is suited for sterilization / autoclaving procedures.
Used For
- Turbidity (optical density) scale:
- CU (concentration units)
- Absorption based monitoring of
- cell growth and bacterial fermentation (Biotech, Pharma)
- algae concentration (Biotech, Pharma, food)
Typical Applications
Fermentation
The ASD12-N absorption probe is designed for the use in pilot scale fermenters or bioreactors including single-use bioreactor bags to precisely measure the growth of microbial or cell cultures as a function of NIR absorption. The ASD12-N is autoclavable and mounts conveniently through any headplate or aseptic connector with a PG13.5 threaded port. The sensor is available in three different insertion depths with 1, 5 or 10 mm optical path lengths. Shorter optical path lengths are typically used for dense cell cultures such as bacterial and yeast cultures. Longer OPL’s are used for less dense cell cultures e.g., mammalian cells and dissolution / precipitation / crystallization reactions.
| ASD12-N | ||
|---|---|---|
| Schematic | 
No window gaskets used |
|
| Measurement | ||
| Measurement principle | 1- Channel Absorption of light | |
| Measurement wavelength(s) | 840 – 910 nm | |
| Detector(s) | 1 silicon photodiode (hermetically sealed) | |
| Measuring Range | any measuring range between:
|
|
| Optical path length | 1, 5 or 10 mm others on request | |
| Calibration | CU (concentration units) application specific calibration | |
| Light source | Hybrid LED (hermetically sealed), 5.4 V DC, 100 mA
typical life span: approx 10 years |
|
| Protection | IP68 | |
| Process Adaption | ||
| Material | wetted parts: stainless steel 1.4435 (SS 316 L) dF < 1 %, BN2
surface: electro-polished Ra < 0,4 μm |
|
| Port connection | Fermenter head plates
diameter:12 mm thread:PG 13.5 |
|
| Port gasket | O-ring 11.00 x 3.00 mm
EPDM (FDA / USP Class VI) |
|
| Insertion depth | 110 mm + OPL
215 mm + OPL 315 mm + OPL others on request |
|
| Process pressure | pressure-free
(+/- 0.5 bar) (+/- 7.25 psi) |
|
| Windows | Sapphire (seal-less) | |
| Installation accessories | Adapter PG 13.5 Variable depth
Adapter M 26 x 1 – PG 13.5 |
|
| Temperature Ratings | ||
| Process temperature | permanent:5 – 50°C (41 – 122°F) | |
| Ambient temperature | operation:0 – 40 °C (32 – 104 °F)
transport:-20 – 70 °C ( -4 – 158 °F) |
|
| Sterilization conditions | Sterilization must be performed outside the process and the ASD must be disconnected from the power source
(autoclaving possible without cable) max. pressure: 4 bar (58 psi) max. temperature: 135 °C (275 °F) (max. 60 min./day) |
|
| Pressure and temperature ratings specified herein may be subject to limitations – see instruction manual. The appropriate choice of material for all wetted parts is the sole responsibility of the user. Data given are subject to changes without prior notice. | ||
